1)i don't have any PE Micro tools
is it any software tool?
2)I have change the PEMicro interface as openSDA embedded Debug -USB port then also i have face same problem
let's find out if the issue is caused by PE Micro driver - not sure which cyclone version do you have - but you can find proper software on PE Micro pages - PEmicro | Experts in Embedded tools for Flash Programming and Development .
Install it and check if your probe is working with PE Micro software. What about user rights? Do you have administrator privileges on your machine?