Hi Adam,
There should without problem for customer to use 16-bit data port with non-multiplexed mode.
When using the non-multiplexed mode, customer need to make sure the Flexbus pin resource is enough for the application.
For using 16-bit data port, the FB_AD0 pin is obsoleted as address pin, it just read the data with 16 bits (2 bytes).
If there exists address non-aligned, there still could read data out, while it will take more cycles (time).
It could read the odd byte and even byte with byte enable signal.
Wish it helps.
Have a great day,
Ma Hui
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