Hi,
regarding the GUI on matlab side, i think it's more a MathWorks question than Freescale. I suggest you contact
MathWorks - MATLAB and Simulink for Technical Computing
regarding to KL25 Virtual RS232 Port problem, if you use FRDM-KL25 board OpenSDA interface to connect board, it also has feature of CDC virtual serial port. you can use it directly to connect GUI with USB interface. it's easier.

can this help you?
Have a great day,
Zhang Jun
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