Hello,
Based on your screenshot, you are attempting to access an inaccessible device. A possible reason is that the USB cable has been disconnected, or the target device is not in programming mode.
The JN5169 will enter UART programming mode if SPIMISO (DO1) pin 22 is low after RESET.
In our Development Kit, this is handled by the FT232R IC.
Best Regards,
Ricardo