Hi@NicoloStrozzi
As you said, your custom protocol does not fit to any standard protocol (I2C),so I think that it is very difficult to use I2C module based on S32K116.
What do you think of the way that simulate the protocol using the General Purpose Input Output(GPIOs) of the microcontroller,It seems that this method is best suited to meet your requirements.
Below file may help you know more about GPIO Simulated I2C protocol(two-wire serial communication).
https://www.nxp.com/search?keyword=AN12841&start=0&category=documents
AN12841-GPIO Simulated I2C for S08: This application note introduces how to implement GPIO simulated I2C as a master based on S08PB/S08PLS.
BR!
Jim,
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