As per datasheet of VR5510 the OTP programming can be done in development phase using NXP GUI and NXP EVB. is there any way to do it on the ECU/RDB2 board used in the development phase?
That's very difficult to do that,please use the programmer EVB board to do this OTP.
For the OTP burn please read page 26 from below link,OTP burn need combine with GUI and socket board to achieve:
UM11586, KITVR5510SKTEVM Evaluation Kit User Guideline-User Manual (nxp.com)