thank you so much , I configure the LST =0B11, the problem is still existing, but I find how to solve it inspired by you, I add a delay() function after eQADC initialization, if the delay time is long enough, U0 will be equal to U1.
then I should calibrate the result later.
for example, 2.5v DC voltage, the accurate result should be 8190, but my sampling result is 9786 before calibrating, is it 9786 a reasonable result before cailibrating? I mean if my PCB board is bad or my voltage source is not good, why the result without calibrating is so rediculous? I have already used many kinds of cheap MCU without ADC calibarating function , the sampling result is in a reasonable range, why MPC5554's result is so strange ?