Hi Marcos,
Thank you for your detail information.
If you want to disable the PIO0_1 ISP function, just configure the CRP mode as NO_ISP by writing data 0x4E69 7370 to flash address 0x0000 02FC.
You can find this description in the user manual Table 372. Code Read Protection options.

Wish it helps you!
If you still have question, please contact me!
Have a great day,
Kerry
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