Hi @Kan_Li ,
I have connected Logic analyser to SDA and SCL lines of SE051C2 secure chip, i'm seeing the below output

Seems like SE05x is not responding, we have verified the power source.. All fine.
Our schematic is routed like below,

Is this setup is fine? or need changes?
Also, i have used SE050ARD evaluation board to verify the program, it is detected the slave address as 0x48
Same way we have connected to SCL and SDA of our custom board, there is no response in i2cdetect command. please refer the image below,

Please help us to solve this issue, we have already took more time.
Thank you.