Hi @OE_
LPC55 USB0 is Full Speed USB port. The default setting of CMPA turns off the USB0 port. Some users may reconfigure CMPA to enable ISP USB0 in order to use ISP USB0 BOOT, but this is not recommended in practice.
It is mentioned in ReferenceManual page 914 that it would be possible to have ISP use P0_22 as VBUS : PIO1_31(if [bit_22] @0x3FC14 is set to 1, use P0_22.
If you want to enable the USB0, as you says, yes, is set to 1. but If you choose this option please be extra careful as you could brick your device.
As says in the manual "The bootloader supports In-System Programming using the USB peripheral. The target is implemented as USB-HID device classes. When transfer data through USB-HID device class, USB-HID does not use framing packets. Instead, the packetization, inherent in the USB protocol itself is used. The ability for the device to NAK Out transfers (until they can be received) provides the required flow control. The built-in CRC of each USB packet provides the required error detection"
So you can use USB1 with Auto ISP mode. . USB1 in ISP mode is identified as a USB input device in the HID section of the device manager.