Hi Kunsen Chen
No, it doesn't mention in this manual.
But according this AN (also can find same in LPC54s018 UM), "Modifying the OTP is a one-time operation and is not reversed", Secure boot enable bit field is one-time bit. Once it is enabled, there is no way to change it back.
in AN12352, flashloader.exe is only for plain image. flashloader.exe can not work for non-plain image if boot enable bit is enabled, thus we can't program the image in the same way.
By checking with the author of AN12352, I can confirm it.
Have a nice day,
Jun Zhang