Here is the detailed analysis: FYI: Baud-rate chosen is 500KBPS for arbitration phase and 2MBPS for Data phase. classical CAN message is used for analysis.
1. CAN Bus has two nodes.
In Normal case where setting TXEN_N low and putting transceiver into Normal State, Transmit Node (PCAN) sends the CAN frame along with recessive ACK bit and monitors the same bit to check that the packet is received by the Node connected to the bus. Once the receiver node receives and then sends the dominant ack bit in the ack slot. This observation can be seen on the below capture. (FYI: Able to receive both Classical CAN, FD-CAN with both extended and standard IDs.

In case of TXEN_N pin setting HIGH, TJA1465B transmitter is disabled. when I send the packet from PCAN transmitter node, receiver node didn't receive the packet, and no Ack bit is sent by the receiver node instead it is sending an active error flag of 6 bits on TX pin of the receiver Node. Because MCAN protocol errors getting generated. the captured picture is pasted below:

2. When CAN bus has three nodes.
In case of TXEN_N pin setting HIGH, TJA1465B transmitter is disabled. when I send the packet from PCAN transmitter node, both the receiver nodes receive the packet, and Ack bit is also sent by the receiver's node.