Let's be precise first: on i.MX53 there's one USB OTG and three USB host ports. There's no reason why you couldn't use all of them at the same time.
However, i.MX53 quick start board design is such that signals from USB OTG controller are connected to two connectors - one is micro USB and other is host connector. Naturally, you can't use these two at the same time. You can use other host controller on i.MX53QSB (connected to USB HOST 1) at the same time with USB OTG, no issue with that.
