A13. Yes, it is possible to use just one lane while selecting SerDes Mux config using a PCIe controller. From the P2040 SERDES options ECI will be setting PCIe2 to use lanes E & F. If you have them pinned out to x2 connector then it will automatically train down to x1 if a x1 device is inserted. If you don't want to use lane F then power lane F down during reset and set SRDSPCCR0[PEX2_CFG] to x1.
It controls Lynx Tx lane reset function for multi-lane protocols. For multi-lane protocols where the lanes are from left to right (PEX, XAUI), it should be set to 1 while for protocols where the lanes are from right to left (SRIO, Aurora), it should be set to 0. For single-lane protocols (SGMII, SATA) it doesn’t matter. It is paired with BnGCRm0[1STLANE], which determines the master source clock lane for a multi-lane protocol (must always be =1 for nominal lane 0 for the protocol, e.g. lane A for PEX on lanes A-D, and =0 for all other lanes).