Thank you your reply. The gray pins are the default, not disable.
Then here is another question.
When I debug SDK "qn908xcdk_wireless_examples_bluetooth_private_profile_server_bm" in my own PCB board, loT toolbox or connectivity QTool cannot find my MCU BLE device (it works fine when I tested it using Design Kit).
I didn't touch any code in SDK, except I add BleAPP_Start() function on main_task() to start task directly after debugging start without pressing a Button1 (because there is no Button1 in my own PCB board).
In my PCB board, I connect RF pin to inductor and antenna as manual said. The below is a schematic.

What I have to do more to wirelessly connect my own QN9080 in my PCB to phone or PC? Do I have to connect RF pin to any other pins? or which position I have to modify in the code?
Thanks,