Hi Alice,
Your screenshot shows the same problem that I am describing, that FC2 is not configured for I2C operation (see the RED box in my image below). Compare those settings with FC4 (the GREEN box in my image) which is properly configured for I2C.

I experimented and found that FC2, FC3, FC5, FC6 and FC7 all show the configuration problem described above. Only FC1 and FC4 can be configured for I2C operation.
Also note that the auto-generated pin_mux.c code for FC2 is completely different from FC4:
const uint32_t port0_pin25_config = (/* Pin is configured as FC4_RTS_SCL_SSEL1 */
IOCON_PIO_FUNC1 |
/* I2C mode */
IOCON_PIO_I2CSLEW_I2C |
/* Input function is not inverted */
IOCON_PIO_INV_DI |
/* Enables digital function */
IOCON_PIO_DIGITAL_EN |
/* Input filter disabled */
IOCON_PIO_INPFILT_OFF |
/* Low drive: 4 mA */
IOCON_PIO_I2CDRIVE_LOW |
/* I2C 50 ns glitch filter enabled */
IOCON_PIO_I2CFILTER_EN);
/* PORT0 PIN25 (coords: 3) is configured as FC4_RTS_SCL_SSEL1 */
IOCON_PinMuxSet(IOCON, 0U, 25U, port0_pin25_config);
const uint32_t port0_pin26_config = (/* Pin is configured as FC4_CTS_SDA_SSEL0 */
IOCON_PIO_FUNC1 |
/* I2C mode */
IOCON_PIO_I2CSLEW_I2C |
/* Input function is not inverted */
IOCON_PIO_INV_DI |
/* Enables digital function */
IOCON_PIO_DIGITAL_EN |
/* Input filter disabled */
IOCON_PIO_INPFILT_OFF |
/* Low drive: 4 mA */
IOCON_PIO_I2CDRIVE_LOW |
/* I2C 50 ns glitch filter enabled */
IOCON_PIO_I2CFILTER_EN);
/* PORT0 PIN26 (coords: 4) is configured as FC4_CTS_SDA_SSEL0 */
IOCON_PinMuxSet(IOCON, 0U, 26U, port0_pin26_config);
IOCON->PIO[0][8] = ((IOCON->PIO[0][8] &
/* Mask bits to zero which are setting */
(~(IOCON_PIO_FUNC_MASK | IOCON_PIO_DIGIMODE_MASK)))
/* Selects pin function.
* : PORT08 (pin 43) is configured as FC2_RXD_SDA_MOSI. */
| IOCON_PIO_FUNC(PIO08_FUNC_ALT1)
/* Select Analog/Digital mode.
* : Digital mode. */
| IOCON_PIO_DIGIMODE(PIO08_DIGIMODE_DIGITAL));
IOCON->PIO[0][9] = ((IOCON->PIO[0][9] &
/* Mask bits to zero which are setting */
(~(IOCON_PIO_FUNC_MASK | IOCON_PIO_DIGIMODE_MASK)))
/* Selects pin function.
* : PORT09 (pin 44) is configured as FC2_TXD_SCL_MISO. */
| IOCON_PIO_FUNC(PIO09_FUNC_ALT1)
/* Select Analog/Digital mode.
* : Digital mode. */
| IOCON_PIO_DIGIMODE(PIO09_DIGIMODE_DIGITAL));