Maybe others will also have the same problem, update the following reference calculation formula for others' reference.
50mV would be represented as (838,860) which is (0000 1100 1100 1100 1100 1100) in binary or (C CCCC) in Hex.
To represent -50mV you need the two’s complement of this number (-838,860), which is (1111 0011 0011 0011 0011 0100) in binary or (F3 3334) In Hex.
250mV would be represented as (4,194,304) which is (0100 0000 0000 0000 0000 0000) in binary or (40 0000) in Hex.
To represent -250mV you need the two’s complement of this number (-4,194,304), which is (1100 0000 0000 0000 0000 0000) or (C0 0000) in Hex.