Hi Prasanna,
PORTx_PCRn[MUX] can be used to select the pin is Peripheral Chip Select or GPIO.
If you want to configure the Pin as GPIO function, then please set MUX=0001

![PORTx_PCRn[MUX].png PORTx_PCRn[MUX].png](/t5/image/serverpage/image-id/87871iCB00A15261E3F6CF/image-size/large?v=v2&px=999)
Best Regards,
Robin
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