Hi Jeffrey,
Sorry for the late reply!
I have request the documents of K81, may receive them within 48 hours.
Have you check the value of PORTx_PCRn[MUX] during debug, whether it select correct alternate function that meet the Signal Multiplexing table?
And have you check the circuit of TWR-POS-K81F board, whether these pins are disconnect by DNP resistances.
Best Regards,
Robin